Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jacob Wagner and the Fall 2016 Missouri Western State University Genetics class
Date created: 2017-02-21 12:00:00
Date modified: 2017-02-21 12:42:21
Pupp promoter mutant - Substitution of C and G to A at 28 and 30
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_J119408_sequence (Version 1) |
Description
The Pupp promoter responds to UTP (see BBa_J100305). This mutant has a substitution of C to A at the -28 and -30 to test the effects of transcription with a different -28 and -30 nucleotide.Notes
NoneSource
Synthetic oligonucleotides cloned into pClone Red (BBa_J119137).| Sequence Annotation | Location | Component / Role(s) |
| Pupp -35 element Substitution of C to A Substitution of G to A Pupp -10 element | 8,13 11,11 13,13 31,36 | feature/promoter promoter feature/mutation sequence_alteration sequence_alteration feature/mutation feature/promoter promoter |