Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Norman Wang
Date created: 2011-02-17 12:00:00
Date modified: 2015-08-31 04:08:30
Chimerabrick Replacement Cassette
| Types | DnaRegion |
| Roles | Temporary sequence_feature |
| Sequences | BBa_J133650_sequence (Version 1) |
Description
Synthesized oligo intended to fix the original Screening Plasmid 3.0 designfrom >MA reporterA NI< reporterB
to reporterA >MA NI< reporterB
The oligos encode Chimerabrick Replacement Cassette insert (cut X N):
X rE sP1 > M A N
Replaces X to N region in current construct (cut X N):
E X > M A B0032 GFP rE sP1 N I < sP2 rE Hr S P
to:
E X rE sP1 > M A N I < sP2 rE Hr S P
Legend:
> = BsmBI
M = MfeI
A = AvrII
N = NheI
I = NsiI
> = BsaI
rE = rnaseE site
sP1 = spacer 1
sp2 = spacer 2
rE = rnaseE site
Hr = mRNA stabilizing hairpin