BBa_J133653

BBa_J133653 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J133653

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Norman Wang

Date created: 2011-07-21 11:00:00

Date modified: 2015-08-31 04:08:30

aadA Cassette + Flanked by TLTerminators (missing RBS)

Details

• Types: DnaRegion
• Roles: sequence_feature; Temporary
• Sequences: BBa_J133653_sequence (Version 1)

Description

KanR gene BBa_P1003 with BsmBI removed, it has duplicated promoter region for kanR since it was amplified from pSB3K5m which is a pSB3K5 derivative.

Flanked with bidirectional translational Terminators BBa_J133422 (prefix) and BBa_B0042 (postfix)

Notes

n/a

Source

http://partsregistry.org/wiki/index.php/Part:BBa_J133003

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J133422	1,12	feature/stop, stop_codon
BBa I14032 P(Lac) IQ	13,49	promoter, feature/promoter
BBa K125520 Aminoglycoside 3"-Adenylyltransferase (aadA1) Gene CDS	56,847	feature/misc, sequence_feature
BBa_B0042	848,859	feature/stop, stop_codon
E81V mutation GAG to GTG	296,298	feature/mutation, sequence_alteration

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J133653/1

Member of these Collections

• //cds/selectionmarker/antibioticresistance
• iGEM Parts Registry