BBa_J133701

BBa_J133701 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J133701

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Norman Wang

Date created: 2011-06-23 11:00:00

Date modified: 2015-08-31 04:08:30

primers tail extension SP for BioBricking

Details

• Types: DnaRegion
• Roles: primer; Primer
• Sequences: BBa_J133701_sequence (Version 1)

Description

Extend your cloning primers with BioBrick Prefix cutting sites (SpeI-NotI-PstI) by concatenating this sequence on the 3' end. Sequence is designed to minimize primer homodimers with itself, and heterodimer with BBa_J133700.

Make sure to order the primer in the correct 5'->3' orientation and the correct strand for the reverse primer!

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
SpeI	2,7	feature/misc, sequence_feature
NotI	9,16	sequence_feature, feature/misc
PstI	16,21	sequence_feature, feature/misc
3 extra bases for efficient PstI cutting	22,24	sequence_feature, feature/misc

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J133701/1

Member of these Collections

• iGEM Parts Registry