| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_J15102_sequence (Version 1)
|
Description
Escherichia coli lacZ' coding sequence encoding the first 76 amino acids of LacZ (beta-galactosidase). This is sufficient to complement the lacZ-delta-M15 mutation found in commonly used laboratory strains of E. coli, thus allowing blue-white selection. It also can be used to effect a pH drop in the presence of lactose, as in the arsenic biosensor entered by the University of Ediburgh team for iGEM2006.
Notes
Codon 77, TGC, was changed to TAA to truncate the coding sequence.
Source
Escherichia coli BS. The sequence was obtained from gi:146575.
