Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris French
Date created: 2007-07-12 11:00:00
Date modified: 2015-08-31 04:08:32
lacZ' coding sequence ending in TGA
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_J15103_sequence (Version 1) |
Description
This is the same Escherichia coli lacZ' coding sequence as in BBa_J15102, encoding the N-terminal 76 amino acids of LacZ (beta galactosidase), except that it ends with TGA rather than the recommended TAATAA. It was mistakenly incorporated into several later constructs where we had intended to use BBa_J15012. It works exactly the same, but does not fully conform to the Registry recommendations for coding sequences.Notes
Codon 77, normally TGC, was altered to TGA to truncate the sequence.Source
Eschericia coli BS. The sequence was derived from gi:146575.| Sequence Annotation | Location | Component / Role(s) |
| lacZ' start lacZ' end lacZ' silent mutation codon 58 | 1,231 1,3 229,231 174,174 | feature/cds CDS start_codon feature/start stop_codon feature/stop point_mutation feature/s_mutation |