BBa_J153003

BBa_J153003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J153003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniel Camsund / Hsinho Huang

Date created: 2012-02-03 12:00:00

Date modified: 2015-08-31 04:08:32

Ptrc1O-driven GFP reporter

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_J153003_sequence (Version 1)

Description

Uses the strong Ptrc1O promoter (link) to generate untagged GFPmut3b (link).

Usage

This strong GFP generator was used to test for the replication of the broad-host-range shuttle vector pPMQAK1 (link) in various cyanobacteria (Huang etal 2010) (link). This reporter carried on pPMQAK1 has also been used as a reference construct for free cytoplasmic GFP in cyanobacterial protein localization studies (Camsund etal 2011) (link).

Notes

None

Source

This Ptrc1O-driven GFP reporter was assembled from Ptrc1O (link) and an intermediate GFP construct (Part:BBa_I13504).

Components

Access	Instance	Definition
public	BBa_J153001	BBa_J153001
public	BBa_I13504	BBa_I13504

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J153001	1,63	BBa_J153001
BBa_I13504	72,946	BBa_I13504

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J153003/1

Member of these Collections

• iGEM Parts Registry