BBa_J15401

BBa_J15401 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J15401
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris French
Date created: 2007-07-12 11:00:00
Date modified: 2015-08-31 04:08:32

ars promoter + rbs + arsR



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_J15401_sequence (Version 1)

Description

The Escherichia coli chromosomal ars promoter followed by the arsR gene. This is essentially a remade version of the natural condition: see Cai, J., and DuBow, M.S. 1996. Expression of the Escherichia coli chromosomal ars operon. Canadian Journal of Microbiology 42, 662-671; Diorio, C., Cai, J., Marmor, J., Shinder, R., and DuBow, M.S. 1995. An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in Gram negative bacteria. Journal of Bacteriology 177, 2050-2056. This biobrick is thus a self-regulating promoter unit which is repressed in the absence of arsenate or arsenite.

Notes

No issues.

Source

The rbs is synthetic, the other parts are from Escherichia coil K12, see X80057 (gi:510824).

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J15401/1