Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris French
Date created: 2010-01-22 12:00:00
Date modified: 2015-08-31 04:08:33
spac promoter with lacI
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_J15506_sequence (Version 1) |
Description
This is the spac promoter, a hybrid of a Bacillus subtilis phage promoter with the operator sequence of the E. coli lac promoter, along with the repressor-encoding gene lacI. Genes added after this should be inducible with IPTG in either E. coli or B. subtilis.Notes
No special considerations.Source
The spac promoter is from vector pVK168, lacI from E. coli JM109.| Access | Instance | Definition |
| public public public | BBa_J15503 BBa_J15001 BBa_J15504 | BBa_J15503 BBa_J15001 BBa_J15504 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J15503 BBa_J15001 BBa_J15504 | 1,284 293,302 309,1394 | BBa_J15503 BBa_J15001 BBa_J15504 |