V0200

BBa_J176121 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J176121
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brady Laughlin
Date created: 2011-10-17 11:00:00
Date modified: 2015-08-31 04:08:35

V0200



    • Details

    Types
    DnaRegion

    Roles
    plasmid_vector

    Plasmid_Backbone

    Sequences BBa_J176121_sequence (Version 1)

    Description

    FRT-marked mam. transfection vector (from Invitrogen pcDNA5 FRT/TO); no promoter; amp, hygro

    Notes

    TBA

    Source

    TBA

    Sequence Annotation Location Component / Role(s)
    3t3 2.0
    NotI SpeI
    BGH
    BGH
    FRT
    Hygromycin resistance
    SV40 early polyadenylation signal
    pUC Origin
    Ampicillin resistance
    bla
    		205,228
    305,335
    430,447
    436,660
    943,980
    998,1990
    2150,2280
    2663,3336
    3481,4341
    4342,4440
    		primer_binding_site feature/primer_binding
    engineered_region feature/BioBrick
    primer_binding_site feature/primer_binding
    polyA_site feature/polya
    feature/misc sequence_feature
    feature/cds CDS
    polyA_site feature/polya
    sequence_feature feature/misc
    CDS feature/cds
    promoter feature/promoter

    igem#experience
    Works
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J176121/1
     

    Attachments

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