Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brady Laughlin
Date created: 2011-10-20 11:00:00
Date modified: 2015-08-31 04:08:35
pcDNA3.1+ puro
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_J176122_sequence (Version 1) |
Description
pcDNA3.1+ puro/ ΔpCMV, based on pcDNA3.1+; no promoter; amp, puroNotes
TBASource
TBA| Sequence Annotation | Location | Component / Role(s) |
| DD122B pCMV remnant SpeI EcoRI PstI NotI XbaI STOP in frame w/ XbaI STOP in frame w/ XbaI BGH BGH DD123B f1 origin SV40 Puromycin resistance SV40 Ampicillin resistance bla | 186,203 232,249 250,324 320,322 329,331 336,353 342,566 344,362 612,1040 1045,1389 1393,1992 2206,2335 3533,4393 4394,4492 | primer_binding_site feature/primer_binding feature/misc sequence_feature feature/BioBrick engineered_region feature/stop stop_codon feature/stop stop_codon primer_binding_site feature/primer_binding polyA_site feature/polya primer_binding_site feature/primer_binding sequence_feature feature/misc feature/promoter promoter CDS feature/cds feature/polya polyA_site feature/cds CDS promoter feature/promoter |