BBa_J176125

BBa_J176125 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J176125
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brady Laughlin
Date created: 2011-10-21 11:00:00
Date modified: 2015-08-31 04:08:35

NLS-pcDNA3.1+



Types
DnaRegion

Roles
plasmid_vector

Plasmid_Backbone

Sequences BBa_J176125_sequence (Version 1)

Description

NLS-pcDNA3.1+, pcDNA3.1+ with Eco/Spe NLS BioBrick inserted into Eco/Xba; amp, neo

Notes

TBA

Source

TBA

Sequence Annotation Location Component / Role(s)
CMV promoter
T7 promoter priming
MCS
BGH reverse priming
BGH polyadenylation sequence
f1 origin
SV40 promoter/ origin
SV40 early polyA signal
pUC origin
AMP resistance gene
NLS - T0100
X/S mixed site
MCS (continued)
BB prefix
Neomycin R
source
232,819
863,882
895,951
1025,1042
1031,1255
1301,1729
1734,2077
3107,3237
3620,4290
4435,5431
973,993
994,999
1000,1013
952,972
2139,2933
1,5431
sequence_feature feature/misc
feature/primer_binding primer_binding_site
sequence_feature feature/misc
primer_binding_site feature/primer_binding
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
feature/misc sequence_feature
feature/dna sequence_feature
sequence_feature feature/misc
feature/misc sequence_feature
feature/misc sequence_feature
feature/misc sequence_feature
feature/dna sequence_feature
feature/misc sequence_feature
igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J176125/1