BBa_J176129

BBa_J176129 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J176129
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brady Laughlin
Date created: 2011-10-25 11:00:00
Date modified: 2015-08-31 04:08:35

pcVN



Types
DnaRegion

Roles
Plasmid_Backbone

plasmid_vector

Sequences BBa_J176129_sequence (Version 1)

Description

VP64-NLS-pcDNA3.1+, pcDNA3.1+ with Eco/Spe NLS BioBrick inserted into Eco/Xba; amp, neo

Notes

TBA

Source

TBA

Sequence Annotation Location Component / Role(s)
CMV promoter
T7 promoter priming
MCS
BGH reverse priming
BGH polyadenylation sequence
f1 origin
SV40 promoter/origin
SV40 early polyA signal
pUC origin
Amp resistance gene
NLS - T0100
X/S mixed site
MCS (continued
BB prefix
Neomycin R
VP64
X/S mixed site
source
232,819
863,882
895,951
1181,1198
1187,1411
1457,1885
1890,2233
3263,3393
3776,4446
4591,5587
1129,1149
1150,1155
1156,1169
952,972
2295,3089
973,1122
1123,1128
1,5587
feature/misc sequence_feature
primer_binding_site feature/primer_binding
sequence_feature feature/misc
feature/primer_binding primer_binding_site
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
feature/misc sequence_feature
feature/misc sequence_feature
sequence_feature feature/dna
feature/misc sequence_feature
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/dna
feature/misc sequence_feature
feature/misc sequence_feature
feature/misc sequence_feature
igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J176129/1