BBa_J179000

BBa_J179000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J179000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jara Radeck

Date created: 2013-12-03 12:00:00

Date modified: 2015-08-31 04:08:35

pBS1C: Bacillus subtilis vector, amyE integration, CAM-resistance

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_J179000_sequence (Version 1)

Description

This vector integrates into the amyE-site of Bacillus subtilis via double recombination. The two recombination sites flank a standard BioBrick multiple cloning site (with RFP-cassette), as well as a chloramphenicol resistance cassette (5 ??g/ml). The backbone does not contain the restriction sites EcoRI, XbaI, SpeI, PstI, NgoMIV nor AgeI.
The vector is replicative in Escherichia coli and provides resistance to ampicillin (100 ??g/ml).

Before transformation into B. subtilis, this vector should be linearized with ScaI.

Notes

This is one of three empty vectors with compatible integration loci and resistance cassettes to allow combination of up to three constructs of choice in one single strain.

Source

pDG1731

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
cat	358,1008	CDS, feature/cds
amyE-back	1379,2407	feature/misc, sequence_feature
bla	4361,5221	CDS, feature/cds
amyE-front	5546,6066	feature/misc, sequence_feature
ori	3587,4201	sequence_feature, feature/misc

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J179000/1

Member of these Collections

• //chassis/multihost
• //chassis/prokaryote/bsubtilis
• //chassis/prokaryote/ecoli
• //plasmid/chromosomalintegration
• //plasmidbackbone/assembly
• //plasmidbackbone/operation
• iGEM Parts Registry
• igem_plasmid_vectors