BBa_J179001

BBa_J179001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J179001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jara Radeck

Date created: 2013-12-20 12:00:00

Date modified: 2015-08-31 04:08:35

pBS2E: Bacillus subtilis vector, lacA integration, MLS resistance

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_J179001_sequence (Version 1)

Description

This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the lacA locus and can be selected with erythromycin + lincomycin (MLS). It has a ampicillin resistance for cloning in E.coli.

Notes

Compatible with pBs1C and pBs4S in one strain.

Source

This backbone is a BioBricked version of the B. subtilis vector pAX01.
This BioBrick is part of the LMU 2012 igem project BacillusBioBrickBox

Reference: H??rtl et al.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
lacA back	124,531	sequence_feature, feature/misc
bla	747,1607	feature/cds, CDS
ori	1758,2381	sequence_feature, feature/misc
lacA front	4078,4593	feature/misc, sequence_feature
erm	4891,5628	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J179001/1

Member of these Collections

• //chassis/multihost
• //chassis/prokaryote/bsubtilis
• //chassis/prokaryote/ecoli
• //plasmid/chromosomalintegration
• //plasmidbackbone/operation
• iGEM Parts Registry
• igem_plasmid_vectors