Types | DnaRegion
|
Roles | Primer
primer
|
Sequences | BBa_J18910_sequence (Version 1)
|
Description
Primer for backbone linearization of RFC 25 construction vectors. Aligns to the RFC 25 prefix but pointing outward into the backbone rather than insert.
Annealing temperature: 68 C (in Phusion polymerase buffer)
Notes
optimized for use with BBa_J18911 (reverse suffix)
Source
designed