T7start

BBa_J18912 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J18912

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Raik Gruenberg

Date created: 2010-01-26 12:00:00

Date modified: 2015-08-31 04:08:36

T7 promoter + RBS + START

Details

• Types: DnaRegion
• Roles: ribosome_entry_site; RBS
• Sequences: BBa_J18912_sequence (Version 1)

Description

Strong T7 promoter and RBS including start codon for protein expression in E. coli or in-vitro.
Part is provided in RFC 25 assembly format.

Notes

• sequence is taken from the commonly used pET3a expression vector

• deleted XbaI site between prom. and RBS

• T7 promoter sequence (1-19) equals the one in BBa_I712074

• Conservative choice of part boundaries and modifications -- the part's size could probably be reduced.

Source

pET3a expression vector

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start	81,83	feature/start, start_codon

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J18912/1

Member of these Collections

• //plasmid/expression/t7
• iGEM Parts Registry