FLAG

BBa_J18914 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J18914

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Raik Gruenberg

Date created: 2010-01-26 12:00:00

Date modified: 2015-08-31 04:08:36

1xFlag tag

Details

• Types: DnaRegion
• Roles: Tag; tag
• Sequences: BBa_J18914_sequence (Version 1)

Description

A FLAG (DYKDDDDK) antigen epitope tag used for marking proteins of interest. Proteins carrying this tag can be easily detected using commercial western blotting reagents.

See also: [[BBa_J64005]]

Hopp et al. (1988) A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification. http://www.nature.com/nbt/journal/v6/n10/abs/nbt1088-1204.html

Notes

• gene synthesis

• codon optimized for E. coli

Source

gene synthesis

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J18914/1

Member of these Collections

• iGEM Parts Registry