TEVsite

BBa_J18918 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J18918

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Raik Gruenberg

Date created: 2010-01-26 12:00:00

Date modified: 2015-08-31 04:08:36

TEV cleavage site (E. coli)

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_J18918_sequence (Version 1)

Description

TEV cleaves the following AA sequence with high specificity:

* Glu-Asn-Leu-Tyr-Phe-Gln↓Gly

but:

* Glu-Asn-Leu-Tyr-Phe-Gln↓Ser

is also reported

Note, the part suggested by the Voigt lab, also contains 2 additional 1xGly flanks for increased accessibility.

See also:

* BBa_I712077 (TEV N-term)

* BBa_I712078 (TEV C-term)

* BBa_I712016 (myri+TEV site, Slovenia team igem2007)

* BBa_J64007 (Dan Widmaier, Voigt lab)

References

=====

Dougherty et al. (1989): Molecular genetic analysis of a plant virus polyprotein cleavage site: a model. PMID: 2669323

Notes

• gene synthesis

• codon optimized for E. coli

Source

gene synthesis

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J18918/1

Member of these Collections

• //proteindomain/cleavage
• iGEM Parts Registry