preSCsite

BBa_J18919 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J18919

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Raik Gruenberg

Date created: 2010-01-26 12:00:00

Date modified: 2015-08-31 04:08:36

preScission protease cleavage site (E. coli-optimized)

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_J18919_sequence (Version 1)

Description

cleavage site for PreScission??? protease.

preScission protease cleavage site

(PreScission??? protease is a genetically engineered form of human rhinovirus 3C protease with a GST fusion, allowing for facile cleavage and purification of GST-tagged proteins along with protease removal after recombinant protein cleavage. Enables low-temperature cleavage of fusion proteins containing the eight-residue recognition sequence.)

cleavage site:

* Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro

References

========

Walker et al. (1994): Efficient and rapid affinity purification of proteins using recombinant fusion proteases. PMID: 7764949

Notes

• gene synthesis

• codon optimized for E. coli

Source

gene synthesis

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J18919/1

Member of these Collections

• //proteindomain/cleavage
• iGEM Parts Registry