ZipE34

BBa_J18923 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J18923

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Raik Gruenberg

Date created: 2010-01-26 12:00:00

Date modified: 2015-08-31 04:08:36

E34(I) strong heterodimerizing leucine zipper

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_J18923_sequence (Version 1)

Description

Engineered Leu zipper based on VBP B-ZIP domain that forms strong parallel heterodimers with R34.

Interaction with R34:

• Kd = 6.1 nM

References

======

• Acharya et al. (2002) A heterodimerizing leucine zipper coiled coil system for examining the specificity of a position interactions: amino acids I, V, L, N, A, and K. PMID: 12450375

• Bashor et al (2008) Using engineered scaffold interactions to reshape MAP kinase pathway signaling dynamics. PMID: 18339942

Notes

• gene synthesis

• codon optimized for E. coli

Source

gene synthesis

literature

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J18923/1

Member of these Collections

• //proteindomain/binding
• iGEM Parts Registry