ZipR34

BBa_J18924 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J18924

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Raik Gruenberg

Date created: 2010-01-26 12:00:00

Date modified: 2015-08-31 04:08:36

R34 heterodimerizing leucine zipper

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_J18924_sequence (Version 1)

Description

Engineered Leu zipper based on VBP B-ZIP domain that forms medium-weak parallel heterodimers with variants E34(X). This zipper corresponds to RR34(A) in Acharya et al and R34 in Bashor et al. Bashor et al. used the three different pairs to tune the affinity of their MAPK feedback device.

Interaction with E34(X) variants:

• E34(I): Kd = 6.1 nM = J18923

• E34(V): Kd = 41 nM

• E34(N): Kd = 810 nM

References

• Acharya et al. (2002) A heterodimerizing leucine zipper coiled coil system for examining the specificity of a position interactions: amino acids I, V, L, N, A, and K. PMID: 12450375

• Bashor et al (2008) Using engineered scaffold interactions to reshape MAP kinase pathway signaling dynamics. PMID: 18339942

Notes

• gene synthesis

• codon optimized for E. coli

Source

gene synthesis

literature

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J18924/1

Member of these Collections

• //proteindomain/binding
• iGEM Parts Registry