Lov2

BBa_J18927 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J18927

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Raik Gruenberg

Date created: 2010-01-26 12:00:00

Date modified: 2015-08-31 04:08:36

phot1-LOV2

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_J18927_sequence (Version 1)

Description

The second LOV domain of Arabidopsis phototropin 1 shows light-induced transient (30s) homo-dimerization between a ground state and an excited monomer (Nakasone et al. 2006). LOV2 is predominantly monomeric at concentrations below 100microM but turns dimeric above. Funnily, the high-conc. dimer state experiences photo-dissociation instead. Either the excitation follows a different course from the dimer starting state or the excitation event itself pushes the complex apart.

(CRG-internal ID: rg0090)

References

• Nakasone et al. 2006 PMID: 16679373

• Freddolino, Peter L.; Dittrich, Markus; Schulten, Klaus (2006) Dynamic Switching Mechanisms in LOV1 and LOV2 Domains of Plant Phototropins PMID: 16935961

Notes

The fragment used by Nakasone et al. is 475K - 578G. The core domain 449E - 586R has reportedly lower stability at RT (aggregation).

• gene synthesis

• codon optimized for E. coli

Source

literature

gene synthesis

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J18927/1

Member of these Collections

• //proteindomain/binding
• iGEM Parts Registry