Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Raik Gruenberg
Date created: 2010-01-26 12:00:00
Date modified: 2015-08-31 04:08:36
mCerulean CFP
| Types | DnaRegion |
| Roles | polypeptide_domain Protein_Domain |
| Sequences | BBa_J18930_sequence (Version 1) |
Description
Cerulean GFP is an optimized FRET donor molecule that was rationally designed by Piston and co-workers to eliminate the excited-state heterogeneity of ECFP. In ECFP, a single-exponential fit to the fluorescence lifetime is not feasible due to multiple components, whereas Cerulean exhibits essentially homogeneous excited-state decay kinetics, rendering this protein useful for lifetime imaging... Live cell imaging experiments have demonstrated that Cerulean GFP undergoes highly efficient FRET to yellow acceptor molecules (25, 26). Additionally, Cerulean exhibits more than twice the brightness of ECFP and CyPet, emitting light at fluorescence intensities similar to Citrine (20).References
- Rizzo MA, Springer GH, Granada B, Piston DW. (2004) An improved cyan fluorescent protein variant useful for FRET. PMID: 14990965
Notes
- amino acid sequence taken from a pAG304GAL-Cerulean-ccdB from LindQuist lab
- borders trimmed to match the construct used for PDB 2Q57 (last 9 AA are not resolved in PDB)
- structure starts with MSK, this construct starts with MVSK but the M has been skipped for this Biobrick
- gene synthesis
- codon optimized for E. coli
Source
literaturegene synthesis