BBa_J202000

BBa_J202000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J202000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jiyeon Park
Date created: 2012-07-18 11:00:00
Date modified: 2015-08-31 04:08:36

PBAD mutant#1



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_J202000_sequence (Version 1)

Description

This is mutant of PBAD (BBa_I13453).

Notes

In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part lacks araO2 site which is essential for araC repressor function, araC acts as an activator in the presence of L-arabinose.

Source

This mutant was created by random mutagenesis.

Sequence Annotation Location Component / Role(s)
araI1
araI2
araC transcription start
Mutation
40,56
61,77
112,112
57,57
feature/binding non_covalent_binding_site
feature/binding non_covalent_binding_site
feature/start start_codon
feature/mutation sequence_alteration
igem#experience None  
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_J202000/1