BBa_J202001

BBa_J202001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J202001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jiyeon Park
Date created: 2012-07-18 11:00:00
Date modified: 2015-08-31 04:08:36

PBAD Mutant#2



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_J202001_sequence (Version 1)

Description

This is PBAD (BBa_I13453) mutant. -95 relative to the araC transcriptional start site was changed from C to T. Also, T at -83 was changed to A.

Notes

In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.

Source

This mutant was created by random mutagenesis.

Sequence Annotation Location Component / Role(s)
araI1
araI2
Transcription Start
Mutation 1
Mutation 2
40,56
61,77
112,112
17,17
29,29
feature/binding non_covalent_binding_site
non_covalent_binding_site feature/binding
feature/start start_codon
sequence_alteration feature/mutation
feature/mutation sequence_alteration
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J202001/1