Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jiyeon Park
Date created: 2012-07-25 11:00:00
Date modified: 2015-08-31 04:08:37
PBAD Mutant#3
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_J202002_sequence (Version 1) |
Description
This is PBAD (BBa_I13453) mutant. -71 relative to the araC transcriptional start site was changed from A to G. Also, C at -22 was changed to TNotes
In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.Source
This mutant was created by random mutagenesis.| Sequence Annotation | Location | Component / Role(s) |
| araI1 araI2 Transcription Start Mutation 1 Mutation 2 | 40,56 61,77 112,112 41,41 90,90 | non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding start_codon feature/start sequence_alteration feature/mutation feature/mutation sequence_alteration |