BBa_J202002

BBa_J202002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J202002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jiyeon Park
Date created: 2012-07-25 11:00:00
Date modified: 2015-08-31 04:08:37

PBAD Mutant#3



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_J202002_sequence (Version 1)

Description

This is PBAD (BBa_I13453) mutant. -71 relative to the araC transcriptional start site was changed from A to G. Also, C at -22 was changed to T

Notes

In order to regulate transcription from PBAD, araC binds three different sites called araO2, araI1, and araI2. In the absence of L-arabinose, araC binds both araO2 and araI1 and bends DNA preventing transcription from PBAD. In the presence of L-arabinose, however, araC has a conformational change because of L-arabinose and binds araI1 and araI2, enabling transcription from PBAD. Like the wild-type (I13453), this part does not have araO2 site which is essential for araC repressor function. Therefore, araC acts as an activator in the presence of L-arabinose.

Source

This mutant was created by random mutagenesis.

Sequence Annotation Location Component / Role(s)
araI1
araI2
Transcription Start
Mutation 1
Mutation 2
40,56
61,77
112,112
41,41
90,90
non_covalent_binding_site feature/binding
non_covalent_binding_site feature/binding
start_codon feature/start
sequence_alteration feature/mutation
feature/mutation sequence_alteration
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J202002/1