Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jiyeon Park
Date created: 2012-07-26 11:00:00
Date modified: 2015-08-31 04:08:37
Ptet Mutant#5
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_J202035_sequence (Version 1) |
Description
This is Ptet (BBa_R0040) mutant. Based on Sizemore et al. 1990. Quantitative analysis of Tn10 Tet repressor binding to a complete set of tet operator mutants. Nucleic Acids Research. 18. 2875-2880, position 4 was changed from T to C.Notes
Mutants were designed based on in vivo assay results (Sizemore et al. 1990. Quantitative analysis of Tn10 Tet repressor binding to a complete set of tet operator mutants. Nucleic Acids Research. 18. 2875-2880).Source
This mutant was generated by synthetic oligonucleotides.| Sequence Annotation | Location | Component / Role(s) |
| Mutation Mutation Mutation Mutation O2 O2 | 6,6 14,14 31,31 39,39 1,19 26,44 | feature/mutation sequence_alteration sequence_alteration feature/mutation sequence_alteration feature/mutation feature/mutation sequence_alteration feature/operator operator operator feature/operator |