BBa_J23017

BBa_J23017 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J23017
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Will Bosworth
Date created: 2006-07-23 11:00:00
Date modified: 2015-08-31 04:08:39

RLambda traG knockout (graveyarded)



    • Details

    Types
    DnaRegion

    Roles
    chromosome

    Cell

    Description

    R plasmid with traG knockout

    Notes

    JL8 Forward oligo to knockout traG-r:
    atgaagaaccgaaacaacgccgtggggccacagatacgggcgaaagtgCaggctggagctgcttcgaag
    JL9 Reverse oligo to knockout traG-r:
    tcatatcgtgatcccctccccttcctcgacggtggccgtctggattgggaaGtagccatggtccatatg

    Source

    PCR JL8/JL9 on pKD3 (~1000bp, dPN1, Neb2)
    Electroporate into pKD46xRlambda

    igem#experience
    Fails
     
    igem#sampleStatus
    No part sequence
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J23017/1
     

    Attachments

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