Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Karmella Haynes
Date created: 2006-10-27 11:00:00
Date modified: 2015-08-31 04:08:45
KanB : RBSrev
| Types | DnaRegion |
| Roles | Intermediate engineered_region |
| Sequences | BBa_J31010_sequence (Version 1) |
Description
Kanamyin resistance backwards, followed by RBS backwardsNotes
Kan was PCR-amplified with primers designed to introduce a SpeI site to the left and an XbaI site to the right (reversing the normal order of these two BioBrick cut sites. The product was cut with SpeI and XbaI and ligated into S/X cut pSB1A2. The source for this vector was pSB1A2 carrying RFP. After cloning, we used a S/X digest to check for insertion in the desired oreitnation (reverse). Clones where Kan did not excise inserted in the wrong way (failure to cut is due to S/X mixed sites) and clones where Kan excised successfully inserted in the desired orientation (restoration of S and X sites).Source
Parts BBa_J31002 and BBa_J44001| Access | Instance | Definition |
| public public | BBa_J31002 BBa_J44001 | KanR Backw BBa_J44001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J31002 BBa_J44001 | 1,816 825,839 | KanR Backw BBa_J44001 |