BBa_J31011

BBa_J31011 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_J31011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Karmella Haynes

Date created: 2006-10-28 11:00:00

Date modified: 2015-08-31 04:08:45

RBS-RFP reverse

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_J31011_sequence (Version 1)

Description

BBa_J31010 was created by PCR amplification of the RBS-RFP portion of BBa_I13507.

Notes

The primers were designed to add PstI-NotI-SpeI to the left and XbaI-NotI-EcoRI to the right (reversal of standard BioBrick cloning sites) so that this part could be cloned in the reverse orientation. Placement of a promoter (reverse) to the right of this part should convey RFP expression.

Source

BBa_I13507

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
mRFP 1	4,678	feature/cds, CDS
B0034	685,696	feature/BioBrick, engineered_region
misc	689,692	sequence_feature, feature/misc

Other Properties

• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J31011/1

Member of these Collections

• iGEM Parts Registry