BBa_J329003

BBa_J329003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J329003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eric Leaman
Date created: 2015-06-21 11:00:00
Date modified: 2015-08-31 04:08:46

Simple Quorum Sensing System Expressing YFP



Types
DnaRegion

Roles
engineered_region

Signalling

Sequences BBa_J329003_sequence (Version 1)

Description

This simple part should function as a quorum sensing circuit. It is meant as an improvement over BBa_J329000, which seems to produce too much LuxI, thus keeping the cells in the induced QS state all the time. eYFP is used as a reporter to take advantage of BBa_I13263, as opposed to performing several ligations required to use GFP.

Notes

BBa_K516011 was used since it contains BBa_B0031 instead of BBa_B0034 in front of C0061, as constitutive expression of LuxI seemed too high when BBa_B0034 was used.

Source

N/A

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J329003/1