Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris French
Date created: 2006-10-12 11:00:00
Date modified: 2015-08-31 04:08:46
E. coli chromosomal ars promoter with arsR repressor gene
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_J33201_sequence (Version 1) |
Description
This part consists of the promoter of the E. coli JM109 chromosomal arsenic detoxification operon (ars operon), including the ArsR repressor binding site and the arsR gene encoding the arsR repressor protein, together with its ribosome binding site. Addition of any other genes to the 3' end of this part will result in their expression being dependent on the presence of sodium arsenate or sodium arsenite. Arsenite or arsenite anion binds to the repressor protein ArsR, resulting in inability to repress the promoter. Based on our experiments, a concentration of 1 micromolar sodium arsenate in LB is sufficient for essentially full expression, though this will vary according to conditions.Notes
Note that this sequence includes the arsR gene encoding the ArsR repressor protein, which is thus negatively autoregulated. No additional parts are required for arsenate-induced expression from this part. In principle, this part should also function in hosts other than E. coli.Source
The DNA was derived by PCR using genomic DNA from E. coli JM109. Primers were designed based on the sequence reported by Diorio et al (1995), J. Bacteriol 177 (8), 2050-2056, Genbank accession X80057, GI:510824. The sequence given here is derived by sequencing the biobrick construct.| Sequence Annotation | Location | Component / Role(s) |
| ArsR binding site -35 sequence -10 sequence rbs arsR | 36,54 57,62 80,85 108,112 119,472 | operator feature/operator feature/promoter promoter promoter feature/promoter ribosome_entry_site feature/rbs feature/cds CDS |