Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris French
Date created: 2006-10-12 11:00:00
Date modified: 2015-08-31 04:08:46
E. coli ars promoter, arsR gene and lacZ
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_J33203_sequence (Version 1) |
Description
This biobrick contains the promoter region of the Escherichia coli chromosomal arsenic detoxification opern, including the arsR gene encoding the ArsR repressor protein required for inducibility of this promoter. This is fused to a lacZ' minigene encoding the N-terminal 77 amino acid residues of LacZ, sufficient to complement the lacZ-delta-M15 mutation. When introduced into an E. coli host strain carrying the lacZ-delta-M15 mutation, this construct results in lacZ expression in the presence of arsenate or arsenite anion. LacZ expression can be detected in various ways. This construct was designed for the detection of arsenate and arsenite in water samples.Notes
Since this construct includes the arsR gene encoding the ArsR repressor which regulates the ars promoter, it requires no other promoter-specific functions. However, it will only give LacZ activity in a host expressing the E. coli lacZ-delta-M15 gene.Source
The ars promoter and arsR gene are derived from E. coli JM109 chromosomal DNA. The lacZ' minigene is derived from E. coli BL21 chromosomal DNA, since JM109 does not possess an intact lacZ gene.| Access | Instance | Definition |
| public public | BBa_J33201 BBa_J33202 | BBa_J33201 BBa_J33202 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J33201 BBa_J33202 | 1,518 527,769 | BBa_J33201 BBa_J33202 |