BBa_J36835

BBa_J36835 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J36835

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Perry Tsai

Date created: 2006-10-28 11:00:00

Date modified: 2015-08-31 04:08:47

Lipoprotein signal peptide

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_J36835_sequence (Version 1)

Description

This codes for the 20-amino-acid signal peptide and the 1st nine amino acids of lipoprotein. When fused with another protein downstream, the signal peptide should target the protein to the outer membrane of E. coli, facing the periplasm.

This part was fused with OmpA(aa46-66) and OmpA(aa46-159) in trying to express streptavidin protein on the outer surface of E. coli.

Notes

There is no spacer nucleotide between the part and the SpeI restriction site, so that after BioBricks assembly, the mixed site is 6 base pairs, and the reading frame is maintained between protein domains.

Source

E. coli K12 genome

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J36835/1

Member of these Collections

• iGEM Parts Registry