BBa_J37023

BBa_J37023 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J37023

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: John Chattaway

Date created: 2006-07-26 11:00:00

Date modified: 2015-08-31 04:08:48

Immunologically Tagged aiiA

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_J37023_sequence (Version 1)

Description

This part is identical to the part BBa_C0060 http://partsregistry.org/wiki/index.php/Part:BBa_C0060 but we have added an epitope tag to the protein. http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Antibody_Explorer/Spotlights/Antibodies_To_Epitope_Tags.html

This should allow us to accurately mesure the concentration of this protein using ELISA assays and purify and handle the protein.

This is a work in progress and more data will be added when it is finished

Notes

The FLAG had to be added at the 5' end of the sequence this was done using PCR

Source

http://partsregistry.org/wiki/index.php/Part:BBa_C0060

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Epitope Tag	1,27	feature/tag, tag
AiiA protein	27,777	CDS, feature/protein
Degredation Tag	777,810	feature/misc, sequence_feature

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J37023/1

Member of these Collections

• iGEM Parts Registry