| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_J40004_sequence (Version 1)
|
Description
This is a fusion of Jun to the beta-autotransporter domain of the immunoglobulin A (IgA) protease of Neisseria gonorrhoea (IgA beta) and to the N-terminus of yellow fluorescent protein (YFP). The IgA beta should target the protein to the cell surface, and the Jun should bind Fos. If the bound Fos contains a C-terminus of a fluorescent protein, fluorescence is expected to result.
Notes
The sequence contained an EcoRI site that was removed by converting it to a Mun I site using oligo-based mutagenesis. Three Pst I sites are still found within the gene and will be removed at a later date by site-specific mutagenesis.
Source
Jun-IgA beta provided by LA Fernandez, Department of Microbial Biotechnology, Madrid. The YFP N-terminus was inserted into this plasmid as a PCR fragment; sequence determined by Hu and Kerppola, Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis. Nat Biotechnol. 2003 May;21(5):539-45
