BBa_J52030

BBa_J52030 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_J52030

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jelka Pohar

Date created: 2006-10-14 11:00:00

Date modified: 2015-08-31 03:54:04

dnMyD88-GFP-PEST191

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_J52030_sequence (Version 1)

Description

dnMyD88-linker-GFP-linker-PEST191 is a part where dnMyD88 (important protein in signaling cascade through TLR4 and TLR5 receptor) is linked to GFP (reporter) and PEST191 sequence that leads towards degradation of protein linked with this sequence. It's estimated half-time is 2h. Both linkers have the same sequence and are 6 aminoacid long protein linker.

Notes

Part made by PCR overlap extension. Has mutations and may not be working.

Source

dnMyD88-
GFP-
PEST191-

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
dnMyD88-GFP-PEST191	1,1656	feature/BioBrick, engineered_region
dnMyD88	1,417	CDS, feature/cds
linker	418,435	feature/protein, CDS
GFP	436,1153	CDS, feature/cds
linker	1153,1170	feature/protein, CDS
PEST191-degradation tag	1171,1656	tag, feature/tag

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J52030/1

Member of these Collections

• iGEM Parts Registry