Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Anderson
Date created: 2006-07-30 11:00:00
Date modified: 2015-08-31 02:02:59
EX-Ptet-S-rbsRFP-P "RFP reporter"
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_J61002_sequence (Version 1) |
Description
Plasmid for construction of promoter basic parts. Insertion of a promoter element between XbaI and SpeI sites results in a RFP reporter while retaining the ability to do biobrick assembly. Part J61002 is the tet promoter variant of the plasmid.Notes
N/ASource
PCR ca1020F/R on pSB1A2-I13521 (900bp, SpeI/PstI)Sub into pSB1A2-I13522 (SpeI/PstI)
Product is pBca1020, will be an iGEM number ultimately
ca1020F Forward SpeI oligo for X-rbsRFP-P plasmid
gcactACTAGTgaaagaggagaaatactagatggc
ca1020R Reverse PstI oligo for X-rbsRFP-P plasmid
ccggactgcagcggccgcttctagtatataaacg
| Sequence Annotation | Location | Component / Role(s) |
| beta-lactamase (ampR, bla) promoter mRFP BBa_B0015 ColE1 origin beta-lactamase ampicillin resistance marker rrnB terminator pBR322 origin BioBrick prefix SpeI PstI NotI RBS BBa_B0034 RBS Verification reverse (VR) primer binding site Verification forward (VF2) primer binding site Ambiguity; should be a T | 2728,2756 27,707 741,869 1049,1731 1826,2686 752,795 1052,1671 2927,2948 2,7 885,890 878,885 9,20 9,20 948,967 2809,2828 2941,2941 | feature/promoter promoter CDS feature/cds engineered_region feature/BioBrick feature/dna sequence_feature CDS feature/cds stem_loop feature/stem_loop feature/dna sequence_feature feature/dna sequence_feature feature/dna sequence_feature sequence_feature feature/dna sequence_feature feature/dna ribosome_entry_site feature/rbs engineered_region feature/BioBrick primer_binding_site feature/primer_binding primer_binding_site feature/primer_binding feature/mutation sequence_alteration |