TnP

BBa_J61023 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J61023

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: John Anderson

Date created: 2006-09-20 11:00:00

Date modified: 2015-08-31 02:02:59

Tn5 Transposase [Tnp1-PN] (with PstI and NotI)

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_J61023_sequence (Version 1)

Description

Tn5 transposase-expressing cassette from pRL. Cassette includes a complete promoter/rbs/orf cassette. The promoter is Ptet, but not derived from r0040. There are PstI and NotI sites in this part, so care must be taken when doing assembly not to use those sites.

pSB1A2-Bca1014

Notes

There are PstI and NotI sites in this part, so care must be taken when doing assembly not to use those sites.

Source

PCR ca1014F/R on pRL27 (1763 bp, EcoRI/SpeI)

Sub into pSB1A2-I13521 (EcoRI/SpeI)

Product is Bca1014 *internal PstI and NotI sites

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J61023/1

Member of these Collections

• iGEM Parts Registry