BBa_J64004

BBa_J64004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J64004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Dan Widmaier

Date created: 2008-01-15 12:00:00

Date modified: 2015-08-31 01:56:26

DH domain constitutively active

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_J64004_sequence (Version 1)

Description

This protein coding frame is a Guanine Exchange Factor (GEF) domain from human eukaryotic with point mutations to make it constitutively active. This protein can be secreted at high titer via the Salmonella T3S using the Voigt Lab secretion circuit.

Notes

This part contains 2 PstI sites and is incompatible with biobrick standard assembly

Source

Human Genomic DNA library clone with 2 point mutations

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
DH Domain	7,612	feature/protein, CDS
HindIII Restriction Site	1,6	feature/misc, sequence_feature
NotI Restriction Site	613,621	feature/misc, sequence_feature

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J64004/1

Member of these Collections

• iGEM Parts Registry