| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_J64007_sequence (Version 1)
|
Description
The Tobacco Etch Virus (TEV) protease cleavage recognition sequence. This DNA sequence encodes an amino acid sequence recognized by TEV protease (ENLYFQS) for efficient cleavage. The sequence is flanked by glycines on the ends to increase flexibility and accessibility to the protease. After proteolytic processing the C-terminal fragment retains only an S residue, however by the design of this part it will leave an SG dipeptide which becomes the new N-terminus of the mature polypeptide.
Notes
This part was codon optimized for expression in E.Coli.
Source
Synthetically created from oligonucleotide synthesis
