BBa_J64010

BBa_J64010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J64010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jeffrey J Tabor

Date created: 2007-01-15 12:00:00

Date modified: 2015-08-31 01:56:26

lasI promoter

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_J64010_sequence (Version 1)

Description

Promoter driving the lasI gene in Pseudomonas aeruginosa.
Activated by LasR+3OC12HSL and not RhlR+C4HSL (Seed et al., 1995)
~4x stronger than the lasB promoter (BBa_R0079; Seed et al., 1995).
Switch point ~0.1nM 3OC12HSL (Seed et al., 1995)

Notes

Promoter sequence cropped to 4nt upstream of Las box according to example of Whiteley and Greenberg, J. Bac, 2001

Source

Genomic sequence of Pseudomonas aeruginosa

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Las box	5,20	operator, feature/operator
+1	53,53	start_codon, feature/start

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J64010/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //direction/forward
• //function/cellsignalling/lasi
• //promoter
• //regulation/positive
• //rnap/prokaryote/ecoli/sigma70
• iGEM Parts Registry