BBa_J64100

BBa_J64100 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_J64100

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jeffrey J Tabor

Date created: 2007-04-02 11:00:00

Date modified: 2015-06-15 12:42:46

Medium copy ColE1 BioBrick vector

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_J64100_sequence (Version 1)

Description

This plasmid is based on pProTetE333 (clontech). A SpeI site was removed via an A-T mutation at position 1166 on the original plasmid, resulting in pProTetE333b. The G00100-G00101 fragment of pSB4A3-1 which includes P1010 between a biobricks multiple cloning site which itself is flanked by + strand transcriptional terminators was then cloned between the XhoI and AvrII sites of pProTetE333b. The resulting plasmid carries a ColE1 origin of replication, a Chloramphenicol resistance marker, the biobricks MCS and the ccdAB death cassette.

Notes

None

Source

pProTetE333 (clontech) and biobricks parts

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
G00100	6,25	primer_binding_site, feature/primer_binding
prefix	124,145	feature/BioBrick, engineered_region
P1010	146,820	engineered_region, feature/BioBrick
suffix	821,841	feature/BioBrick, engineered_region
G00101	977,996	primer_binding_site, feature/primer_binding
ColE1 origin	1130,1812	sequence_feature, feature/misc
chloramphenicol resistance marker	1939,2598	feature/misc, sequence_feature
G00101	1029,1048	feature/primer_binding, primer_binding_site

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J64100/1

Member of these Collections

• //plasmidbackbone/assembly
• //plasmidbackbone/copynumber/medium
• //plasmidbackbone/operation
• iGEM Parts Registry
• igem_plasmid_vectors