Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: laura lavery
Date created: 2007-03-24 12:00:00
Date modified: 2015-05-08 01:08:17
LasR/LasI Inducible & RHLR/RHLI repressible Promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_J64712_sequence (Version 1) |
Description
This part is similar to BBa_R0079 but has an added binding site for RHLR/RHLI in between the -35 and -10 region. Thus this promoter is induced by LasR/LasI but repressed by RHLR/RHLI binding.Notes
The natural spacer between the -35 and -10 is 14bp and the natural O1 site for RHLR/RHLI is 20bp long. There was not much over lap of the -35 or the -10 with this sequence, so it was nessicary to chop off some of the binding site sequence in order to maintain the correct spacing and preserve the -35 and -10 sequence. The binding site sequence is missing a tcc on is 5' end and a t on its 3' end.Source
Rust L. et. al., "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Journal of Bacteriology, 1996.| Sequence Annotation | Location | Component / Role(s) |
| LasR/LasI O2 LasR/LasI O1 RHLR/RHLI -35 -10 | 46,64 106,125 125,141 117,122 140,145 | operator feature/operator operator feature/operator feature/binding non_covalent_binding_site feature/misc sequence_feature sequence_feature feature/misc |