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BBa_J64714

BBa_J64714 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J64714

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: laura lavery

Date created: 2007-03-24 12:00:00

Date modified: 2015-05-08 01:08:17

RHLR/RHLI Inducible (LasR/LasI repressible) positive feedback production of C4 HSL

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Details

Types	DnaRegion
Roles	Project engineered_region
Sequences	BBa_J64714_sequence (Version 1)

Description

This construct will produce more C4 HSL when the circuit is given an inital amount of C4 HSL and when RHLR is present in the system. This construct will also produce GFP as a reporter when expressed.

Notes

Project goals

Source

J64800 was designed using R0071 and the binding site for LasR/LasI was taked from R0079. The rest of the parts were already in the registry.

Components

Access	Instance	Definition
public public public public public public public	BBa_J64800 BBa_B0034 BBa_J64718 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012	BBa_J64800 BBa_B0034 BBa_J64718 BBa_B0034 GFP BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J64800 BBa_B0034 BBa_J64718 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012	1,53 62,73 80,688 697,708 715,1434 1443,1522 1531,1571	BBa_J64800 BBa_B0034 BBa_J64718 BBa_B0034 GFP BBa_B0010 BBa_B0012

Other Properties

igem#experience	None
igem#sampleStatus	Not in stock
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_J64714/1