BBa_J64804

BBa_J64804 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J64804
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sai Duriseti
Date created: 2007-03-19 12:00:00
Date modified: 2015-05-08 01:08:17

The promoter region (inclusive of regulator binding sites) of the B. subtilis RocDEF operon



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_J64804_sequence (Version 1)

Description

This is the reported promoter region from the Bacillus subtilis RocDEF operon as taken from the DBTBS website (http://dbtbs.hgc.jp/). Base 1 to base 21 contains the binding site for the binding factor, RocR. The sequence, TATGCAAAAGAATTTTGCACT, reportedly contains the consensus sequence for binding of this factor. Base 42 to base 62 contains another binding site for the RocR binding factor, with ATATCAGAATGTTTTTGCACC as the binding consensus for this sequence. Bases 113-135 outline the binding site of the binding factor AhrC (CTTGCATTTATATAAAGGGAAAG). Bases 96 to 135 outline the promoter region for this operon (CTTGATTTGGCACAGAACTTGCATTTATATAAAGGGAAAG).

Notes

This sequence only outlines the reported promoter region as ascertained from the online resource described above, and may lack nucleotides not described by the operon analysis used.

Source

Information was obtained from: http://dbtbs.hgc.jp/COG/prom/rocDEF.html.

Sequence Annotation Location Component / Role(s)
RocR Binding Site
RocR Binding Site
Promoter
AhrC
1,21
42,62
96,135
113,131
feature/binding non_covalent_binding_site
non_covalent_binding_site feature/binding
feature/promoter promoter
feature/binding non_covalent_binding_site
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J64804/1