BBa_J64975

BBa_J64975 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J64975

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kristopher Kuchenbecker

Date created: 2007-03-23 12:00:00

Date modified: 2015-05-08 01:08:17

anti-glnG RNAi

Details

• Types: DnaRegion
• Roles: mature_transcript_region; RNA
• Sequences: BBa_J64975_sequence (Version 1)

Description

Cognate RNAi for glnG from -40 to +24. Last 10 bases on 3' end are not involved in binding with glnG mRNA, but rather serve as a binding site for an additional RNAi

Notes

Wanted an RNAi that would overlap the RBS and start codon for glnG. As an additional constraint on the design, also wanted to allow regulation of the cytosolic levels of this RNAi via an additional RNAi. This was accomplished by extending the 3' end with a G/C-rich region.

Source

E. coli K-12

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J64975/1

Member of these Collections

• iGEM Parts Registry