Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sai Duriseti
Date created: 2007-03-25 11:00:00
Date modified: 2015-05-08 01:08:18
OmpR-P strong binding, regulatory region for Team Challenge03-2007
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_J64981_sequence (Version 1) |
Description
Contains the F1 consensus site from the OmpF regulator. Contains a LacI repressor consensus binding site between the -35 and -10 portions of a T7 core promoter region, and a TetR consensus binding site upstream of the -35 site.Notes
The weaker binding repressor binds upstream of the core promoter (upstream of -35), while the stronger binds inbetween the -35 and -10.Source
Information for creation of this construct came from the following sorces:Yoshida et al. JBC 2006
Frank et al. JMB 1997
Rong et al. PNAS 1998
Merabet et al. Biochemistry 1995
Su et al. JBC 1998
Kedracka-Krok et al. JPC 1999
Kleinschmidt et al. Biochemistry 1988
Butler et al. JBC 1982
Jorgensen et al. JBC 1991
Hillen et al. JMB 1984
Head et al. JMB 1998
| Sequence Annotation | Location | Component / Role(s) |
| Strong OmpR TetR Consensus T7 RNA Polymerase Recognition LacI Consensus -35 -10 | 1,20 21,39 40,82 46,65 40,45 66,70 | non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding feature/promoter promoter non_covalent_binding_site feature/binding sequence_feature feature/misc feature/misc sequence_feature |