Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tom Knight
Date created: 2008-10-19 11:00:00
Date modified: 2015-05-08 01:08:20
MF-plasmid-ori
| Types | DnaRegion |
| Roles | plasmid Plasmid |
| Sequences | BBa_J70023_sequence (Version 1) |
Description
This part is a cassette containing the PE gene from the pSci2 plasmid of Spiroplasma citri. It is reported to convey replication ability for the group 1 spiroplasmas. The part contains the replication origin, the promoter, and gene for the PE protein.Notes
No native restriction sites. Added biobrick ends.Source
Genbank AJ969070| Sequence Annotation | Location | Component / Role(s) |
| forward PCR priimer fwd2 dnaA binding dnaA binding imperfect repeat dnaA binding imperfect repeat dnaA binding rbs pe Iteron Iteron Low GC region reverse PCR primer | 1,27 177,201 328,336 394,402 458,469 472,480 579,589 763,771 816,820 830,1483 1481,1491 1501,1511 1646,1822 1854,1877 | primer_binding_site feature/primer_binding primer_binding_site feature/primer_binding sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc feature/misc sequence_feature ribosome_entry_site feature/rbs CDS feature/cds sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/primer_binding primer_binding_site |